What is tissue culture(TC)?
Tissue culture is a method of growing and multiplying plants from small pieces of plant tissue under controlled, sterile conditions.
The tissue is placed into a nutrient-rich culture medium that provides what it needs to develop and grow.
With the right conditions, one small piece of a plant can eventually become multiple new plants.
1.Choose plant material
We select a suitable part of the plant tissue, called an explant, depending on the species and the goal of the propagation process.
2.Prepare the culture medium
The explant needs a suitable culture medium to grow in. It contains water, nutrients, sugars and other components that support the plant's development under controlled conditions.
Before introducing the plant tissue, the culture medium, tools, vessels and working environment need to be sterilized. This helps prevent bacteria and fungi from growing alongside the plant and taking over the culture.
3.Sterilization
4.Introduce the explant
The sterilized explant is carefully placed onto the prepared culture medium under sterile conditions. From this point, the plant tissue can begin adapting to the medium and developing new growth.
5.Establish the culture
After introduction, the explant needs time to adapt to its new environment and begin growing. During this stage, we monitor the culture for healthy development and signs of contamination.
6.Multiplication
Once healthy growth is established, new shoots or other suitable plant material can be separated and transferred onto fresh culture medium. By repeating this process, a single starting explant can produce many new plants.
7.Rooting
Once enough shoots have developed, they can be encouraged to produce roots. This gives each young plant the ability to take up water and nutrients independently and prepares it for life outside the culture vessel.
8.Growing in the vessel
The young plants continue developing inside their culture vessels under controlled conditions. They are given time to develop strong roots and shoots before they are ready to leave the in-vitro environment.
9.Acclimatization
